Antioxidant capacity of proteins and hydrolysates from the liver of newborn piglets, and their inhibitory effects on steatosis in vitro
Research background. Non-alcoholic steatohepatitis is a potentially progressive hepatic disorder that can lead to end-stage liver disease and hepatocellular carcinoma. The inhibitory effects of proteins and hydrolysates from the liver of newborn piglets on hepatic steatosis in oleic acid-induced hep...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01001116119/Details |
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| Matična publikacija: |
Food technology and biotechnology (Online) 58 (2020), 4 ; str. 455-464 |
| Glavni autori: | Zhang, Ruilin (Author), Chen, Jian, Yin, Lasheng, Zhang, Xuewu |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.17113/ftb.58.04.20.6657 Food technology and biotechnology (Online) Hrčak |
| LEADER | 04646naa a22003974i 4500 | ||
|---|---|---|---|
| 001 | NSK01001116119 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20211202145549.0 | ||
| 006 | m d | ||
| 007 | cr|||||||||||| | ||
| 008 | 211022s2021 ci ad |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.17113/ftb.58.04.20.6657 | |
| 035 | |a (HR-ZaNSK)001116119 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b hrv |b eng | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 66 |2 2011 | |
| 100 | 1 | |a Zhang, Ruilin |4 aut |9 HR-ZaNSK | |
| 245 | 1 | 0 | |a Antioxidant capacity of proteins and hydrolysates from the liver of newborn piglets, and their inhibitory effects on steatosis in vitro |h [Elektronička građa] / |c Ruilin Zhang, Jian Chen, Lasheng Yin, Xuewu Zhang. |
| 300 | |b Ilustr., graf. prikazi. | ||
| 504 | |a Bibliografija: 27 jed. | ||
| 504 | |a Summary ; Sažetak. | ||
| 520 | |a Research background. Non-alcoholic steatohepatitis is a potentially progressive hepatic disorder that can lead to end-stage liver disease and hepatocellular carcinoma. The inhibitory effects of proteins and hydrolysates from the liver of newborn piglets on hepatic steatosis in oleic acid-induced hepatocellular carcinoma (HepG2) cells were investigated in vitro. Experimental approach. The extracted proteins from the liver of newborn piglets were hydrolysed with papain, pepsin, trypsin and Alcalase. Based on the comparison of different enzyme digestion conditions, a protein hydrolysis protocol was established to obtain hydrolysates with lipid-lowering effect. Results and conclusions. Trypsin-digested liver protein hydrolysate from newborn piglets exhibited strong antioxidant activity and good inhibitory effects against lipogenesis and cholesterol accumulation in HepG2 cells at the concentration of 150 μg/mL, with a triglyceride decrease of (43±3) % and cholesterol decrease of (31±5) %, compared with model group induced with 0.75 mM oleic acid. The addition of trypsin digested liver protein hydrolysate from newborn piglets (300 μg/mL) decreased alanine aminotransferase and aspartate aminotransferase activities and increased superoxide dismutase activity. Novelty and scientific contribution. This study demonstrated that the trypsin-digested liver protein hydrolysate from newborn piglets has a potential preventive effect against non-alcoholic fatty liver disease in its early stage, and a potential use as the modulator of lipid overaccumulation in form of food supplements. | ||
| 520 | |a Pozadina istraživanja. Nealkoholni steatohepatitis je progresivna bolest jetre koja može dovesti do terminalnog stadija bolesti i pojave hepatocelularnog karcinoma. Provedeno je ispitivanje in vitro učinka jetrenih proteina odojka i njihovih hidrolizata na inhibiciju steatoze u stanicama hepatocelularnog karcinoma induciranog oleinskom kiselinom (HepG2). Eksperimentalni pristup. Proteini su izdvojeni iz jetre odojaka i hidrolizirani pomoću papaina, pepsina, tripsina i alkalaze. Usporedbom različitih uvjeta enzimske razgradnje stvoren je protokol hidrolize proteina za dobivanje hidrolizata koji smanjuju količinu masnoća u krvi. Rezultati i zaključci. Hidrolizati jetrenih proteina odojka dobiveni pomoću 150 μg/mL tripsina imali su izražen antioksidacijski učinak i dobro svojstvo inhibicije lipogeneze i nakupljanja kolesterola u HepG2 stanicama, te su smanjili udjel triglicerida za (43±3) % i kolesterola za (31±5) %, u usporedbi s kontrolnom skupinom stanica karcinoma induciranog pomoću 0,75 mM oleinske kiseline. Dodatkom 300 μg/mL hidrolizata proteina dobivenog pomoću tripsina smanjila se aktivnost alanin aminotransferaze i aspartat aminotransferaze, a povećala aktivnost superoksid dismutaze. Novina i znanstveni doprinos. U radu je dokazano da hidrolizat jetrenih proteina odojka dobiven pomoću tripsina može u ranoj fazi prevenirati nealkoholnu bolest masne jetre, te se primijeniti kao dodatak hrani za regulaciju prekomjernog nakupljanja lipida. | ||
| 653 | 0 | |a Jetra odojka |a Hidrolizat proteina |a Antioksidacijska aktivnost |a Oleinska kiselina |a Lipidi | |
| 700 | 1 | |a Chen, Jian |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Yin, Lasheng |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Zhang, Xuewu |4 aut |9 HR-ZaNSK | |
| 773 | 0 | |t Food technology and biotechnology (Online) |x 1334-2606 |g 58 (2020), 4 ; str. 455-464 |w nsk.(HR-ZaNSK)000484955 | |
| 981 | |b Be2021 |b B01/21 | ||
| 998 | |b dalo2112 | ||
| 856 | 4 | 0 | |u https://doi.org/10.17113/ftb.58.04.20.6657 |
| 856 | 4 | 0 | |u https://www.ftb.com.hr/images/pdfarticles/2020/October-December/FTB-58-455.pdf |y Food technology and biotechnology (Online) |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/251262 |y Hrčak |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |