Pyruvate decarboxylase activity assay in situ of different industrial yeast strains
Summary: Cytoplasmic pyruvate decarboxylase (PDC, EC 4.1.1.1) is one of the key enzymes of yeast fermentative metabolism. PDC is the first enzyme which, under anaerobic conditions, leads to decarboxylation of pyruvate with acetaldehyde as the end product. The aim of this study is to develop a suitab...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01000735960/Details |
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| Matična publikacija: |
Food technology and biotechnology 47 (2009), 1 ; str. 96-100 |
| Glavni autor: | Berlowska, Joanna (-) |
| Ostali autori: | Kregiel, Dorota (-), Ambroziak, Wojciech |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
Food Technology and Biotechnology |
| LEADER | 02441caa a2200289 ir4500 | ||
|---|---|---|---|
| 001 | NSK01000735960 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20121016120115.0 | ||
| 007 | ta | ||
| 008 | 100507s2009 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)738584 | ||
| 035 | |a (HR-ZaNSK)000735960 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 042 | |a croatica | ||
| 080 | |a 664 |2 MRF 1998. | ||
| 100 | 1 | |a Berlowska, Joanna | |
| 245 | 1 | 0 | |a Pyruvate decarboxylase activity assay in situ of different industrial yeast strains / |c Joanna Berłowska, Dorota Kregiel, Wojciech Ambroziak. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 17 jed | ||
| 520 | 8 | |a Summary: Cytoplasmic pyruvate decarboxylase (PDC, EC 4.1.1.1) is one of the key enzymes of yeast fermentative metabolism. PDC is the first enzyme which, under anaerobic conditions, leads to decarboxylation of pyruvate with acetaldehyde as the end product. The aim of this study is to develop a suitable method for PDC activity assay in situ for different industrial yeast strains. Saccharomyces sp. and Debaryomyces sp. yeast strains grew in fermentative medium with 12 % of glucose. Enzymatic assay was conducted in cell suspension treated with digitonin as permeabilisation agent, and with sodium pyruvate as a substrate, at temperature of 30 °C. Metabolites of PDC pathway were detected using gas chromatographic (GC) technique. Various parameters like type and molar concentration of the substrate, minimal effective mass fraction of digitonin, cell concentration, reaction time and effect of pyrazole (alcohol dehydrogenase inhibitor) were monitored to optimize PDC enzymatic assay in situ. In the concentration range of yeast cells from 1⋅10 7 to 1⋅10 8 per mL, linear correlation between the produced acetaldehyde and cell density was noticed. Only pyruvate was the specific substrate for pyruvate decarboxylase. In the presence of 0.05 M sodium pyruvate and 0.05 % digitonin, the enzymatic reaction was linear up to 20 min of the assay. During incubation, there was no formation of ethanol and, therefore, pyrazole was not necessary for the assay | |
| 653 | 0 | |a Piruvat dekarboksilaza |a Kvasac | |
| 700 | 1 | |a Kregiel, Dorota | |
| 700 | 1 | |a Ambroziak, Wojciech | |
| 773 | 0 | |t Food technology and biotechnology |x 1330-9862 |g 47 (2009), 1 ; str. 96-100 |w nsk.(HR-ZaNSK)000174173 | |
| 981 | |b B03/09 | ||
| 998 | |a Luko100507 |c vol2o121016 | ||
| 856 | 4 | 2 | |u http://hrcak.srce.hr/ftb |y Food Technology and Biotechnology |