Effect of haemolysis on an enzymatic measurement of ethanol
Introduction: We investigated the interference of haemolysis on ethanol testing carried out with the Synchron assay kit using an AU680 autoanalyser(Beckman Coulter, Brea, USA).Materials and methods: Two tubes of plasma samples were collected from 20 volunteers. Mechanical haemolysis was performed in...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01001163356/Details |
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| Matična publikacija: |
Biochemia medica (Online) 31 (2021), 1 ; str. 85-92 |
| Glavni autori: | Çat, Abdulkadir (Author), Uçar, Kamil Taha, Gümüş, Alper |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Online pristup: |
https://doi.org/10.11613/BM.2021.010704 Elektronička verzija članka Elektronička verzija članka Elektronička verzija članka |
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| 024 | 7 | |2 doi |a 10.11613/BM.2021.010704 | |
| 035 | |a (HR-ZaNSK)001163356 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
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| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
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| 100 | 1 | |a Çat, Abdulkadir |4 aut |9 HR-ZaNSK | |
| 245 | 1 | 0 | |a Effect of haemolysis on an enzymatic measurement of ethanol |h [Elektronička građa] |c Abdulkadir Çat, Kamil Taha Uçar, Alper Gümüş. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: | ||
| 504 | |a Summary. | ||
| 520 | |a Introduction: We investigated the interference of haemolysis on ethanol testing carried out with the Synchron assay kit using an AU680 autoanalyser(Beckman Coulter, Brea, USA).Materials and methods: Two tubes of plasma samples were collected from 20 volunteers. Mechanical haemolysis was performed in one tube,and no other intervention was performed in the other tube. After centrifugation, haemolysed and non-haemolysed samples were diluted to obtainsamples with the desired free haemoglobin (Hb) values (0, 1, 2, 5, 10 g/L). A portion of these samples was then separated, and ethanol was added tothe separated sample to obtain a concentration of 86.8 mmol/L ethanol. After that, these samples were diluted with ethanol-free samples with thesame Hb concentration to obtain samples containing 43.4, 21.7, and 10.9 mmol/L. Each group was divided into 20 equal parts, and an ethanol testwas carried out. The coefficient of variation (CV), bias, and total error (TE) values were calculated.Results: The TE values of haemolysis-free samples were approximately 2-5%, and the TE values of haemolysed samples were approximately 10-18%. The bias values of haemolysed samples ranged from nearly - 6.2 to - 15.7%.Conclusions: Haemolysis led to negative interference in all samples. However, based on the 25% allowable total error value specified for ethanol inthe Clinical Laboratory Improvement Amendments (CLIA 88) criteria, the TE values did not exceed 25%. Consequently, ethanol concentration can bemeasured in samples containing free Hb up to 10 g/L. | ||
| 700 | 1 | |a Uçar, Kamil Taha |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Gümüş, Alper |4 aut |9 HR-ZaNSK | |
| 773 | 0 | |t Biochemia medica (Online) |x 1846-7482 |g 31 (2021), 1 ; str. 85-92 |w nsk.(HR-ZaNSK)000655372 | |
| 981 | |b Be2021 | ||
| 856 | 4 | 0 | |u https://doi.org/10.11613/BM.2021.010704 |
| 856 | 4 | 0 | |u https://www.biochemia-medica.com/en/journal/31/1/10.11613/BM.2021.010704 |y Elektronička verzija članka |
| 856 | 4 | 0 | |u https://www.biochemia-medica.com/en/journal/31/1/10.11613/BM.2021.010704/fullArticle |y Elektronička verzija članka |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/252079 |y Elektronička verzija članka |