Production of microbial transglutaminase on media made from sugar Cane molasses and glycerol
Summary: Transglutaminase is an enzyme that catalyses an acyl transfer reaction between γ-carboxamide groups of glutaminyl residues and lysine residues in proteins. Due to this property, this enzyme is used for enhancing textural properties of protein-rich food. The transglutaminase used as food add...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01000735798/Details |
|---|---|
| Matična publikacija: |
Food technology and biotechnology 47 (2009), 1 ; str. 19-26 |
| Glavni autor: | Portilla-Rivera, Oscar M. (-) |
| Ostali autori: | Tellez-Luis, Simon J. (-), Leon, Jose A. Ramirez de, Vazquez, Manuel |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
Food Technology and Biotechnology |
| LEADER | 02771caa a2200301 ir4500 | ||
|---|---|---|---|
| 001 | NSK01000735798 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20121016113035.0 | ||
| 007 | ta | ||
| 008 | 100506s2009 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)738422 | ||
| 035 | |a (HR-ZaNSK)000735798 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 042 | |a croatica | ||
| 080 | |a 664 |2 MRF 1998. | ||
| 100 | 1 | |a Portilla-Rivera, Oscar M. | |
| 245 | 1 | 0 | |a Production of microbial transglutaminase on media made from sugar Cane molasses and glycerol / |c Oscar M. Portilla-Rivera, Simon J. Tellez-Luis, Jose A. Ramirez de Leon, Manuel Vazquez. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 26 jed | ||
| 520 | 8 | |a Summary: Transglutaminase is an enzyme that catalyses an acyl transfer reaction between γ-carboxamide groups of glutaminyl residues and lysine residues in proteins. Due to this property, this enzyme is used for enhancing textural properties of protein-rich food. The transglutaminase used as food additive is obtained by microorganisms, mainly by Streptoverticillium ladakanum. On the other hand, sugar cane molasses is a viscous liquid rich in noncrystallized carbohydrates (saccharose, glucose and fructose). In this work, the feasibility of using sugar cane molasses as a carbon source for the production of microbial transglutaminase by Streptoverticillium ladakanum NRRL 3191 has been studied. Carbon sources including sugar cane molasses (60 g of total sugars per L), glycerol (60 g/L) and their mixture in a ratio of 1:1 (30 g/L of each) were evaluated. Time course of microbial growth, transglutaminase activity and carbon source consumption were determined every 24 h during 120 h of fermentations at three agitation speeds (200, 300 or 400 rpm). The results showed that with the increase in agitation speed, the biomass concentration increased up to 8.39 g/L in the medium containing sugar cane molasses alone or the mixture of molasses and glycerol. The highest transglutaminase activity was obtained at 400 rpm in the medium containing a mixture of molasses and glycerol, reaching 0.460 U/mL, while in the medium containing sugar cane molasses alone, the activity was 0.240 U/mL, and using glycerol alone it was 0.250 U/mL. These results show that sugar cane molasses is a suitable medium for transglutaminase production when it is combined with glycerol | |
| 653 | 0 | |a Mikrobna transglutaminaza |a Melasa šećerne trske |a Glicerol | |
| 700 | 1 | |a Tellez-Luis, Simon J. | |
| 700 | 1 | |a Leon, Jose A. Ramirez de | |
| 700 | 1 | |a Vazquez, Manuel | |
| 773 | 0 | |t Food technology and biotechnology |x 1330-9862 |g 47 (2009), 1 ; str. 19-26 |w nsk.(HR-ZaNSK)000174173 | |
| 981 | |b B03/09 | ||
| 998 | |a Luko100506 |c vol2o121016 | ||
| 856 | 4 | 2 | |u http://hrcak.srce.hr/ftb |y Food Technology and Biotechnology |