Aspartic proteases from basidiomycete Clitocybe nebularis
Summary: We have isolated aspartic proteases by affinity chromatography from wild growing basidiomycete Clitocybe nebularis. Pepstatin A sensitive fractions from size exclusion chromatography were subjected to Concanavalin A affinity chromatography. N-terminal sequences of the three bands resolved o...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01000734982/Details |
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| Matična publikacija: |
Croatica chemica acta 82 (2009), 4 ; str. 739-745 |
| Glavni autor: | Sabotič, Jerica (-) |
| Ostali autori: | Popovič, Tatjana, biologinja (-), Brzin, Jože |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
Croatica Chemica Acta |
| LEADER | 01669caa a2200289 ir4500 | ||
|---|---|---|---|
| 001 | NSK01000734982 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20130307104002.0 | ||
| 007 | ta | ||
| 008 | 100428s2009 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)737604 | ||
| 035 | |a (HR-ZaNSK)000734982 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 042 | |a croatica | ||
| 080 | |a 54 |2 MRF 1998. | ||
| 100 | 1 | |a Sabotič, Jerica | |
| 245 | 1 | 0 | |a Aspartic proteases from basidiomycete Clitocybe nebularis / |c Jerica Sabotič, Tatjana Popovič, and Jože Brzin. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 31 jed | ||
| 520 | 8 | |a Summary: We have isolated aspartic proteases by affinity chromatography from wild growing basidiomycete Clitocybe nebularis. Pepstatin A sensitive fractions from size exclusion chromatography were subjected to Concanavalin A affinity chromatography. N-terminal sequences of the three bands resolved on SDS-PAGE showed sequence similarity to the A01.018 group of family A1 aspartic proteases of the MEROPS classification. The diversity of putative aspartic proteases found in Clitocybe nebularis basidiocarp extracts is considerable and shows the great potential of basidiomycetes as a source of unique proteases that could find use in biotechnological applications and drug design | |
| 653 | 0 | |a Aspartatska proteaza |a Clitocybe nebularis |a Afinitetna kromatografija |a Basidiomiceti | |
| 700 | 1 | |a Popovič, Tatjana, |c biologinja | |
| 700 | 1 | |a Brzin, Jože | |
| 773 | 0 | |t Croatica chemica acta |x 0011-1643 |g 82 (2009), 4 ; str. 739-745 |w nsk.(HR-ZaNSK)000001621 | |
| 981 | |b B02/09 | ||
| 998 | |a Luko100428 |c vol2o130307 | ||
| 856 | 4 | 2 | |u http://hrcak.srce.hr/cca |y Croatica Chemica Acta |