Plasma-desorption mass spectrometry as an express diagnostic test for bovine leukaemia
Plasma-desorption mass spectrometry with ionization of 252Californium fission fragments (252Cf-PD MS) was used to develop an express diagnostic test for bovine leukaemia. The test is based on the mass spectrometric parameters of artificial lipid membranes formed on the gold substrate from the lipid...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01001145260/Details |
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| Matična publikacija: |
Veterinarska stanica (Online) 52 (2021), 2 ; str. 159-166 |
| Glavni autori: | Bordunova, Olga (Author), Kovalenko, Lidia, Chernenko, Olexander, Bondarchuk, Larisa, Levchenko, Irina, Izmailova, Nataliy, Cherniavskaya, Tatyana, Chivanov, Vadym |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.46419/vs.52.2.2 Hrčak |
| LEADER | 04189naa a22004334i 4500 | ||
|---|---|---|---|
| 001 | NSK01001145260 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20221122141328.0 | ||
| 006 | m d | ||
| 007 | cr|||||||||||| | ||
| 008 | 220803s2020 ci d |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.46419/vs.52.2.2 | |
| 035 | |a (HR-ZaNSK)001145260 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b hrv | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 636.09 |2 2011 | |
| 100 | 1 | |a Bordunova, Olga |4 aut |9 HR-ZaNSK | |
| 245 | 1 | 0 | |a Plasma-desorption mass spectrometry as an express diagnostic test for bovine leukaemia |h [Elektronička građa] / |c Olga Bordunova, Lidia Kovalenko, Olexander Chernenko, Larisa Bondarchuk, Irina Levchenko, Nataliy Izmailova, Tatyana Cherniavskaya, Vadym Chivanov. |
| 300 | |b Graf. prikazi. | ||
| 504 | |a Bibliografija: 21 jed. | ||
| 504 | |a Abstract ; Sažetak. | ||
| 520 | |a Plasma-desorption mass spectrometry with ionization of 252Californium fission fragments (252Cf-PD MS) was used to develop an express diagnostic test for bovine leukaemia. The test is based on the mass spectrometric parameters of artificial lipid membranes formed on the gold substrate from the lipid fraction of blood cells (mainly lymphocytes), by the self-assembly technique according to the biomimetic principle. Mass spectrometric analysis of artificial lipid membranes obtained from blood plasma of control and leukemic cattle gave a reliable diagnosis of leukaemia. The basis for the diagnosis were the ionization parameters of the fragmentation of lipid molecules from normal and leukemic cell membranes. In leukemic cell membranes, separate dense areas (rafts) 200 – 300 nm in diameter, saturated with cholesterol and phospholipids, are formed. The number of rafts per unit of cell membrane of leukemic cells significantly exceeded the permitted parameters of normal cell membranes. The increased number of rafts in artificial membranes formed in the membranes of leukemic cells was reliably reflected in mass spectra, due to the 180 – 250% increase in intensity of peaks of cholesterol fragment ions in comparison to control cells. | ||
| 520 | |a Korištenjem desorpcije plazme pomoću masene spektrometrije s ionizacijom fragmenata fizije 252kalifornijskih fragmenata fizije (252Cf-PD MS) razvijen je brzi test za dijagnostiku leukemije goveda. Istraživanje se temelji na masenim spektrometrijskim pokazateljima umjetnih lipidnih membrana formiranih na zlatnom supstratu iz lipidne frakcije krvnih stanica (uglavnom limfocita) samo-sastavnom tehnikom prema biomimetičkom principu. Masena spektrometrijska analiza lipidnih umjetnih membrana dobivenih iz krvne plazme kontrolne skupine i skupine goveda oboljelih od leukemije omogućava pouzdanu dijagnozu leukemije. Osnova za dijagnozu bili su pokazatelji ionizacije i fragmentacije lipidnih molekula iz normalnih staničnih membrana i leukemijskih stanica. U membranama leukemijskih stanica formiraju se zasebna gusta područja (tzv. splavi) promjera 200 - 300 nm, zasićena kolesterolom i fosfolipidima. Broj splavi po jedinici stanične membrane leukemijskih stanica znatno je veći od odgovarajućih pokazatelja membrane normalnih stanica. Povećani broj splavi u umjetnim membranama formiranim iz membrana stanica goveda oboljelih s leukemijom pouzdano se odražava u masenim spektrima u povećanju intenziteta vrhunaca iona fragmenata kolesterola za 180 - 250 % u usporedbi sa skupinom kontrolnih goveda. | ||
| 653 | 0 | |a Govedo |a Leukemija |a Kolesterol |a Masena spektrometrija |a Fosfokolin | |
| 700 | 1 | |a Kovalenko, Lidia |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Chernenko, Olexander |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Bondarchuk, Larisa |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Levchenko, Irina |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Izmailova, Nataliy |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Cherniavskaya, Tatyana |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Chivanov, Vadym |4 aut |9 HR-ZaNSK | |
| 773 | 0 | |t Veterinarska stanica (Online) |x 1849-1170 |g 52 (2021), 2 ; str. 159-166 |w nsk.(HR-ZaNSK)000855872 | |
| 981 | |b Be2020 |b B06/20 | ||
| 998 | |b tino2211 | ||
| 856 | 4 | 0 | |u https://doi.org/10.46419/vs.52.2.2 |
| 856 | 4 | 0 | |u http://hrcak.srce.hr/239460 |y Hrčak |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |