Proteinaceous pancreatic lipase inhibitor from the seed of Litchi chinensis
A study of the pancreatic lipase inhibitory activity of a protein from the seed of Litchi chinensis was carried out. Protein was isolated by 70 % ammonium sulphate precipitation followed by dialysis. Lipase inhibitory activity of the protein was evaluated using both synthetic (p-nitrophenyl palmitat...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01001065369/Details |
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| Matična publikacija: |
Food technology and biotechnology (Online) 57 (2019), 1 ; str. 113-118 |
| Glavni autori: | Mhatre, Sveeta V. (Author), Bhagit, Amita A., Yadav, Raman P. |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.17113/ftb.57.01.19.5909 Hrčak Food technology and biotechnology (Online) |
| LEADER | 04142naa a22003974i 4500 | ||
|---|---|---|---|
| 001 | NSK01001065369 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20200714140424.0 | ||
| 006 | m d | ||
| 007 | cr|||||||||||| | ||
| 008 | 200603s2019 ci ad |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.17113/ftb.57.01.19.5909 | |
| 035 | |a (HR-ZaNSK)001065369 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b eng |b hrv | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 663 |2 2011 | |
| 080 | 1 | |a 613 |2 2011 | |
| 100 | 1 | |a Mhatre, Sveeta V. |4 aut |9 HR-ZaNSK | |
| 245 | 1 | 0 | |a Proteinaceous pancreatic lipase inhibitor from the seed of Litchi chinensis |h [Elektronička građa] / |c Sveeta V. Mhatre, Amita A. Bhagit, Raman P. Yadav. |
| 300 | |b Ilustr., graf. prikazi. | ||
| 504 | |a Bibliografija: 30 jed. | ||
| 504 | |a Summary ; Sažetak. | ||
| 520 | |a A study of the pancreatic lipase inhibitory activity of a protein from the seed of Litchi chinensis was carried out. Protein was isolated by 70 % ammonium sulphate precipitation followed by dialysis. Lipase inhibitory activity of the protein was evaluated using both synthetic (p-nitrophenyl palmitate) and natural (olive oil) substrates. Protein at the final concentration of 100 µg/mL was able to inhibit 68.2 % pancreatic lipase on synthetic substrate and 60.0 % on natural substrate. Proteinaceous nature of the inhibitor was determined using trypsinization assay. Pancreatic lipase inhibitory protein was sensitive to 0.05 % trypsin treatment with the loss of 61.9 % activity. IC50 of this proteinaceous pancreatic lipase inhibitor was 73.1 µg/mL using synthetic substrate. This inhibitory protein was sensitive to pH, with the highest inhibitory activity at pH=8.0 and the lowest at pH=3.0. Protein was further analyzed using 10 % non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and, interestingly, it showed the presence of a single band of (61±2) kDa when stained with Coomassie brilliant blue. The isolated protein was finally crystallized to see its homogeneity by batch crystallization method. Crystals were well formed with distinct edges. The isolated protein showed good pancreatic lipase inhibitory activity. | ||
| 520 | |a U radu je ispitana inhibicija lipaze (enzima kojega luči gušterača) pomoću proteina izoliranog iz sjemenki azijske trešnje (Litchi chinensis). Protein je izoliran taloženjem pomoću amonijevog sulfata i dijalizom. Inhibicija lipaze u prisutnosti proteina ispitana je na sintetičkom (pnitrofenil palmitat) i prirodnom (maslinovo ulje) supstratu. Protein je pri koncentraciji od 100 ug/mL inhibirao 68,2 % lipaze na sintetičkom i 60,0 % na prirodnom supstratu. Svojstva inhibitora ispitana su tripsinizacijom. Protein je bio osjetljiv na dodatak 0.05 % tripsina, pri čemu je izgubio 61,9 % aktivnosti. Njegova IGO vrijednost na sintetičkom supstratu bila je 73,1 gg/mL. Također je bio osjetljiv na promjenu pH-vrijednosti, te je najveću aktivnost imao pri pH=8,0, a najmanju pri pH=3,0. Osim toga, protein je ispitan pomoću poliakrilamidne gel elektroforeze u nereducirajućim uvjetima uporabom 10 %-tne otopine natrijevog dodecil sulfata. Nakon obrade bojilom Coomassie brilliant blue zamijećena je prisutnost samo jedne proteinske vrpce od (61±2) kDa. Izolirani je protein naposljetku kristaliziran šaržnom kristalizacijom radi ispitivanja njegove homogenosti. Kristali su bili dobro formirani, s jasno definiranim rubovima. Protein je pokazao dobru aktivnost inhibicije lipaze. | ||
| 653 | 0 | |a Azijska trešnja |a Litchi chinensis |a Sjemenke voća |a Lipaza |a Proteini |a Pretilost | |
| 700 | 1 | |a Bhagit, Amita A. |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Yadav, Raman P. |4 aut |9 HR-ZaNSK | |
| 773 | 0 | |t Food technology and biotechnology (Online) |x 1334-2606 |g 57 (2019), 1 ; str. 113-118 |w nsk.(HR-ZaNSK)000484955 | |
| 981 | |b Be2019 |b B03/19 | ||
| 998 | |b dalo2007 | ||
| 856 | 4 | 0 | |u https://doi.org/10.17113/ftb.57.01.19.5909 |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/218906 |y Hrčak |
| 856 | 4 | 0 | |u http://www.ftb.com.hr/images/pdfarticles/2019/January-March/FTB-57-113.pdf |y Food technology and biotechnology (Online) |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |