Production of β-fructofuranosidase by Arthrobacter sp. and its application in the modification of stevioside and rebaudioside A
Arthrobacter sp. 10137 has been used to produce β-fructofuranosidase (FFase). Sucrose and corn steep powder in an optimized ratio of 10:1 were the best carbon and nitrogen sources for enzyme production in a shake flask. The maximum FFase activity was 26.69 U/mL after 22.5 h in batch culture, and the...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01000736214/Details |
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| Matična publikacija: |
Food technology and biotechnology 47 (2009), 2 ; str. 137-143 |
| Glavni autor: | Xu, Zhong-Wei (-) |
| Ostali autori: | Li, Yu-Qiang (-), Wang, Yong-Hua, Yang, Bo, biotehnolog, Ning, Zhengxiang |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
Food Technology and Biotechnology |
| LEADER | 01945caa a2200325 ir4500 | ||
|---|---|---|---|
| 001 | NSK01000736214 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20131231081147.0 | ||
| 007 | ta | ||
| 008 | 100510s2009 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)738838 | ||
| 035 | |a (HR-ZaNSK)000736214 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 042 | |a croatica | ||
| 080 | |a 664 |2 MRF 1998. | ||
| 100 | 1 | |a Xu, Zhong-Wei | |
| 245 | 1 | 0 | |a Production of β-fructofuranosidase by Arthrobacter sp. and its application in the modification of stevioside and rebaudioside A / |c Zhong-Wei Xu, Yu-Qiang Li, Yong-Hua Wang, Bo Yang, Zheng-Xiang Ning. |
| 246 | 3 | |a Production of beta-fructofuranosidase by Arthrobacter sp. and its application in the modification of stevioside and rebaudioside A | |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 18 jed. | ||
| 520 | 8 | |a Arthrobacter sp. 10137 has been used to produce β-fructofuranosidase (FFase). Sucrose and corn steep powder in an optimized ratio of 10:1 were the best carbon and nitrogen sources for enzyme production in a shake flask. The maximum FFase activity was 26.69 U/mL after 22.5 h in batch culture, and the crude FFase, obtained by ultrafiltration and (NH4)2SO4 fractionation, was purified about 7-fold as measured by specific activity from the crude culture filtrate. The FFase was specific for introduction of a fructose molecule at the C19 position on both the stevioside and rebaudioside A, with high transfructosylating activity of 65 % after 15 h of incubation. | |
| 653 | 0 | |a β-fruktofuranozidaza |a Steviozid |a Rebaudiozid |a Arthrobacter sp. | |
| 700 | 1 | |a Li, Yu-Qiang | |
| 700 | 1 | |a Wang, Yong-Hua | |
| 700 | 1 | |a Yang, Bo, |c biotehnolog | |
| 700 | 1 | |a Ning, Zhengxiang | |
| 773 | 0 | |t Food technology and biotechnology |x 1330-9862 |g 47 (2009), 2 ; str. 137-143 |w nsk.(HR-ZaNSK)000174173 | |
| 981 | |b B03/09 | ||
| 998 | |a Luko100510 |c vol2o121017 | ||
| 856 | 4 | 2 | |u http://hrcak.srce.hr/ftb |y Food Technology and Biotechnology |