Biotransformation of (R)-(+)-lLimonene by the psychrotrophic fungus Mortierella minutissima in H2O2-oxygenated culture
Summary: The aim of the present work is to investigate an integration of a microbial reaction system for the oxidation of limonene using a psychrotrophic strain with an unconventional oxygenation of the culture. The alternative method for increasing the dissolved oxygen concentration in culture medi...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01000736213/Details |
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| Matična publikacija: |
Food technology and biotechnology 47 (2009), 2 ; str. 131-136 |
| Glavni autor: | Trytek, Mariusz (-) |
| Ostali autori: | Fiedurek, Jan (-), Skowronek, Marcin |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
Food Technology and Biotechnology |
| LEADER | 02360caa a2200289 ir4500 | ||
|---|---|---|---|
| 001 | NSK01000736213 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20121017091418.0 | ||
| 007 | ta | ||
| 008 | 100510s2009 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)738837 | ||
| 035 | |a (HR-ZaNSK)000736213 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 042 | |a croatica | ||
| 080 | |a 66 |2 MRF 1998. | ||
| 100 | 1 | |a Trytek, Mariusz | |
| 245 | 1 | 0 | |a Biotransformation of (R)-(+)-lLimonene by the psychrotrophic fungus Mortierella minutissima in H2O2-oxygenated culture / |c Mariusz Trytek, Jan Fiedurek, Marcin Skowronek. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 35 jed | ||
| 520 | 8 | |a Summary: The aim of the present work is to investigate an integration of a microbial reaction system for the oxidation of limonene using a psychrotrophic strain with an unconventional oxygenation of the culture. The alternative method for increasing the dissolved oxygen concentration in culture media for biotransformation of R-(+)-limonene by Mortierella minutissima 01 is based on catalase-mediated decomposition of hydrogen peroxide (H2O2) into oxygen and water. Automated addition of H2O2 into the bioreactor made it possible to keep the dissolved oxygen concentration constant over a range from 5 to 100 % (±2 %). Perillyl alcohol and perillyl aldehyde were the main products of the limonene biotransformation. The amounts of perillyl alcohol produced during H2O2-oxygenated culture of M. minutissima 01 were over 2-fold higher in comparison with classical, stirred aeration. Some factors affecting the biotransformation yield were also investigated. The addition of 0.3 % methanol enhanced 1.4-fold the perillyl alcohol production by M. minutissima 01. A maximum yield of this product (258.1 mg/L) was observed between 2 and 3 days of cultivation in a medium containing 0.5 % substrate at 15 °C, pH=6.0. The bioconversion activity increased over 3.6-fold after optimization of some biotransformation conditions | |
| 653 | 0 | |a Mortierella minutissima |a Vodikov peroksid |a Limonen |a Perilil alkohol | |
| 700 | 1 | |a Fiedurek, Jan | |
| 700 | 1 | |a Skowronek, Marcin | |
| 773 | 0 | |t Food technology and biotechnology |x 1330-9862 |g 47 (2009), 2 ; str. 131-136 |w nsk.(HR-ZaNSK)000174173 | |
| 981 | |b B03/09 | ||
| 998 | |a Luko100510 |c vol2o121017 | ||
| 856 | 4 | 2 | |u http://hrcak.srce.hr/ftb |y Food Technology and Biotechnology |