Lipase mediated isoamyl acetate synthesis in solvent-free system using vinyl acetate as acyl donor
Summary: Synthesis of isoamyl acetate, a flavour ester extensively used in food industry, has been carried out in a solvent-free system. In the present study, an attempt has been made to enhance the isoamyl acetate synthesis yield by transesterification of isoamyl alcohol with vinyl acetate using im...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01000735796/Details |
|---|---|
| Matična publikacija: |
Food technology and biotechnology 47 (2009), 1 ; str. 13-18 |
| Glavni autor: | Kumari, Annapurna (-) |
| Ostali autori: | Mahapatra, Paramita (-), Garlapati, Vijay Kumar, Banerjee, Rintu, Dasgupta, Swagata |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
Food Technology and Biotechnology |
| LEADER | 02260caa a2200313 ir4500 | ||
|---|---|---|---|
| 001 | NSK01000735796 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20180626144959.0 | ||
| 007 | ta | ||
| 008 | 100506s2009 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)738420 | ||
| 035 | |a (HR-ZaNSK)000735796 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 042 | |a croatica | ||
| 080 | |a 664 |2 MRF 1998. | ||
| 100 | 1 | |a Kumari, Annapurna | |
| 245 | 1 | 0 | |a Lipase mediated isoamyl acetate synthesis in solvent-free system using vinyl acetate as acyl donor / |c Annapurna Kumari, Paramita Mahapatra, Vijay Kumar Garlapati, Rintu Banerjee, Swagata Dasgupta. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 32 jed. | ||
| 520 | 8 | |a Summary: Synthesis of isoamyl acetate, a flavour ester extensively used in food industry, has been carried out in a solvent-free system. In the present study, an attempt has been made to enhance the isoamyl acetate synthesis yield by transesterification of isoamyl alcohol with vinyl acetate using immobilized Rhizopus oryzae NRRL 3562 lipase. In the present synthesis, substrates had no inhibitory effect on immobilized lipase. The effects of various reaction parameters on isoamyl acetate synthesis were studied and maximum conversion was achieved at 16 % (by mass per volume) of immobilized lipase, 40 °C and 200 rpm. Under these conditions, 8-hour reaction time was sufficient to reach a high ester conversion of 95 % with 0.5 mol/L of isoamyl alcohol. The structure of the transesterified product was confirmed by infrared and nuclear magnetic resonance spectroscopic studies. Immobilized lipase had Km and vmax values of 306.53 mmol/L and 99 µmol/(h·g) respectively, for isoamyl acetate synthesis in a solvent-free system. | |
| 653 | 0 | |a Prehrambena industrija |a Esterifikacija |a Acetat |a Gljivice |a Sojevi |a Rhizopus oryzae |a Enzimi |a Lipaza |a Imobilizirana lipaza |a Enzimska aktivnost | |
| 700 | 1 | |a Mahapatra, Paramita | |
| 700 | 1 | |a Garlapati, Vijay Kumar | |
| 700 | 1 | |a Banerjee, Rintu | |
| 700 | 1 | |a Dasgupta, Swagata | |
| 773 | 0 | |t Food technology and biotechnology |x 1330-9862 |g 47 (2009), 1 ; str. 13-18 |w nsk.(HR-ZaNSK)000174173 | |
| 981 | |b B03/09 | ||
| 998 | |a Luko100506 |c vol2o121016 |b mili1806 | ||
| 856 | 4 | 2 | |u http://hrcak.srce.hr/ftb |y Food Technology and Biotechnology |