Improved performances of polyacrylamide gel by addition of a new type of polymer
Abstract: The aim of this study was to improve detection of heterozygous samples and loss of heterozygosity using a new type of gel polymer. A total of 60 samples of normal and tumor colon tissue were tested. Elctrophoresis of amplified loci was performed on a standard acrylamide / N,Nmethylenebisac...
| Permalink: | http://skupnikatalog.nsk.hr/Record/nsk.NSK01000716598/Details |
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| Matična publikacija: |
Croatica chemica acta 81 (2008), 3 ; str. |
| Glavni autor: | Korolija, Marina (-) |
| Ostali autori: | Hadžija, Mirko (-), Pavković, Pajica, Kapitanović, Sanja, Popović Hadžija, Marijana |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
CROATICA CHEMICA ACTA |
| LEADER | 02374caa a2200349 ir4500 | ||
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| 001 | NSK01000716598 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20180409161704.0 | ||
| 007 | ta | ||
| 008 | 091020s2008 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)719167 | ||
| 035 | |a (HR-ZaNSK)000716598 | ||
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| 041 | 0 | |a eng |b hrv | |
| 042 | |a croatica | ||
| 080 | |a 54 |2 MRF 1998. | ||
| 080 | |a 575 |2 MRF 1998. | ||
| 100 | 1 | |a Korolija, Marina | |
| 245 | 1 | 0 | |a Improved performances of polyacrylamide gel by addition of a new type of polymer : |b detection of allelic losses / |c Marina Korolija, Mirko Hadžija, Pajica Pavković, Sanja Kapitanović and Marijana Popović Hadžija. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 23 jed. | ||
| 504 | |a Sažetak | ||
| 520 | 8 | |a Abstract: The aim of this study was to improve detection of heterozygous samples and loss of heterozygosity using a new type of gel polymer. A total of 60 samples of normal and tumor colon tissue were tested. Elctrophoresis of amplified loci was performed on a standard acrylamide / N,Nmethylenebisacrylamide gel, and the same gel containing Spreadex polymer, NAB (native acrylamide- bis). Standard gel displayed 36 samples of normal tissue as homozygous. Electrophoresis of the same samples on gel with addition of polymer revealed 12/36 heterozygous samples. Five of 12 matched tumor samples displayed loss of heterozygosity that could not be detected on standard gel, because of its lower resolving power. Electrophoresis on a new polymer presents a simple, inexpensive, reproducible and non-radioactive method for loss of heterozygosity detection, with improved performances such as increased sensitivity, decreased amount of a sample and improved separation of closely spaced bands. Application of the polymer may contribute to a vide variety of diagnostic procedures | |
| 653 | 0 | |a Tumorske stanice |a Heterozigotnost |a Homozigotnost |a Gel-elektroforeza |a Polimeri |a Poliakrilamid |a Dijagnostička točnost | |
| 700 | 1 | |a Hadžija, Mirko | |
| 700 | 1 | |a Pavković, Pajica | |
| 700 | 1 | |a Kapitanović, Sanja | |
| 700 | 1 | |a Popović Hadžija, Marijana | |
| 773 | 0 | |t Croatica chemica acta |x 0011-1643 |g 81 (2008), 3 ; str. |w nsk.(HR-ZaNSK)000001621 | |
| 981 | |b B09/08 |p CRO | ||
| 998 | |a rado091020 |c vol2o120830 |p 1605ARi |a mild1804 | ||
| 856 | 4 | 2 | |u http://public.carnet.hr/ccacaa/ |y CROATICA CHEMICA ACTA |